| Group | Club/Team Name | Team ID | Head Coach |
| A01 | Great River Soccer Club Great River - B16 - Skoog |
0WGRV-GRVBAB16-0085 | Brandon Skoog |
| A02 | Joy of the People Joy of the P - B16 |
0EJOP-JOPBAB16-0005 | n/a |
| A03 | Minneapolis United Soccer Club MUS 16U Boys Samba |
0WMUS-MUSBAB16-0123 | Michael Navarre |
| A04 | Northern Lights Soccer Club Northern Lights Soccer Club - B16 |
0NNLS-NLSBAB16-0343 | Luke Olson |
| A06 | Southeast Soccer Club SES U16B Pumas |
0ESES-SESBAB16-0039 | Nathanael Zell |
| A07 | Austin Youth Soccer Association Austin Boys 16U Black |
0SAUS-AUSBAB16-0003 | Corey Anderson |
| A08 | Alexandria Area Soccer Association Alexandria Area Soccer Association - B16 |
NWALX-ALXBAB16-0053 | n/a |
| A09 | Arrowhead Youth Soccer Association Arrowhead Grand Rapids Area 16UB Black League |
0NAHD-AHDBAB16-2003 | Lisa Fox |
| A10 | Gitchi Gummi Soccer Club Gitchi Gummi 16UB Black League |
0NGGM-GGMBAB16-2036 | Matthew Gram |
| A11 | Worthington FC Worthington - B16 |
SWWFC-WFCBAB16-0001 | n/a |