| Group | Club/Team Name | Team ID | Head Coach |
| A1 | North Oaks Soccer Club NOSC Blast-Tanana - B16 |
0NNOC-NOCDB16-0016 | Ogechukwu Edward |
| A2 | Park Valley United FC Park Valley United FC - B16 |
0WPVU-PVUDB16-1405 | Nicholas Wilson |
| A3 | North Oaks Soccer Club NOSC Blast-Yukon - B16 |
0NNOC-NOCDB16-0017 | Prince Amoako |
| A4 | BOREAL FC BOREAL FC - B16 - 2006 White - Strom |
0NBFC-001DB16-0004 | Damien Klug |
| A5 | CC United Soccer Club CC United Boys 16U Div1 |
0WCCU-CCUDB16-0004 | Keaton Koehler |
| A6 | Keliix-Intra Soccer Club Keliix-Intra Soccer Club - B16 |
0WKLX-KLXDB16-0070 | Jason English |
| A7 | Minnesota Rush Minnesota Rush - B16 |
0SMNR-MNRDB16-0256 | Travis Gransee |