| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Great River Soccer Club Great River Soccer Club - G16 |
0WGRV-GRVDG16-0138 | Ronald Fee |
| A2 | Northern Lights Soccer Club CRU/NLS - G16 |
0NNLS-NLSDG16-2006 | Molly Larson |
| A3 | CC United Soccer Club CC United Girls 16U Div1 |
0WCCU-CCUDG16-0001 | Timothy Klein |
| A4 | Mankato United Soccer Club Mankato Unit - G16 |
SWMNK-MNKDG16-0002 | Kindra Dewanz |
| A6 | Mississippi Valley Soccer Club Mississippi Valley Soccer - G16 |
0SMVS-MVSDG16-0044 | Joseph Czerniak |
| A7 | North Oaks Soccer Club NOSC Blast South-Amazon - G16 |
0NNOC-NOCDG16-0012 | Amanda Boehmer |
| A8 | BOREAL FC BOREAL FC - G16 - 2006 White - Cowie |
0NBFC-001DG16-0002 | Jacob Cowie |